Single Cell Brain Isoforms in Space and Time
summary
Develops long-read single-cell and single-nucleus methods (ScISOr-Seq, SnISOr-Seq) plus spatial Slide-isoform sequencing to map full-length isoforms to cell types and tissue coordinates. Reports a 7.5-fold increase in usable exon-overlapping reads for frozen human cortex by depleting intronic/TSO artifacts, finds distant coordinated exons (but not adjacent ones) are strongly cell-type specific, shows autism-associated exons are among the most cell-type-variable, finds most hippocampus vs PFC isoform differences trace to one cell type, and provides ISOquant plus PacBio/Nanopore error comparisons (≈80% TSS concordance).
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